Coding

Part:BBa_K1214008:Design

Designed by: Alec Spinhirne   Group: iGEM13_UT_Dallas   (2013-09-16)

FruR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promotion may be inducible in the presence of fructose.

Cra (Catabolite repressor activator) has been shown to regulate a wide variety of carbon metabolism genes, mainly those for enzymes involved in catabolism in Escherichia coli.
Shimada, T., K. Yamamoto, and A. Ishihama. "Novel Members of the Cra Regulon Involved in Carbon Metabolism in Escherichia Coli." NCBI. US National Library of Medicine, 29 Nov. 2010. Web. 26 Sept. 2013. <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021228/>.

Source

Isolated from genome of E. coli MG1655

References